Preclinical diagnosis of African swine fever in contact-exposed swine by a real-time PCR assay.
نویسندگان
چکیده
A fluorogenic probe hydrolysis (TaqMan) PCR assay for African swine fever virus (ASFV) was developed and evaluated in experimentally infected swine. This sensitive and specific one-step single-tube assay, which can be performed in 2 h or less, detected viral DNA in tonsil scraping samples 2 to 4 days prior to onset of clinical disease. Thus, the assay would have application for preclinical diagnosis of African swine fever and surveillance and/or emergency management of a disease outbreak.
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عنوان ژورنال:
- Journal of clinical microbiology
دوره 43 1 شماره
صفحات -
تاریخ انتشار 2005